Characterization of Herbal Antifungal Agent, Origanum vulgare against Oral Candida spp. Isolated from Patients with Candida-Associated Denture Stomatitis: An In vitro Study.
Author Information
Bhat V1, Sharma SM2, Shetty V3, Shastry CS4, Rao CV5, Shenoy S6, Saha S7, Balaji S8.
1 Department of Prosthodontics, A B Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be University), Mangalore, Karnataka, India.
2 Department of Oral and Maxillofacial Surgery, ABSMIDS, NITTE (Deemed to be University), Mangalore, Karnataka, India.
3 Department of Microbiology, KSHEMA, NITTE (Deemed to be University), Mangalore, Karnataka, India.
4 Principal, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Mangalore, Karnataka, India.
5 Department of Biotechnology, NMAM Institute of Technology, NITTE (Deemed to be University), Mangalore, Karnataka, India.
6 Department of Microbiology, NITTE (Deemed to be University), Mangalore, Karnataka, India.
7 Department of Pharmacognosy, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Mangalore, Karnataka, India.
8 Department of Prosthodontics, Sri Ramakrishna Dental College, and Hospitals, Coimbatore, Tamil Nadu, India.
Abstract
MATERIALS & METHODOLOGY: Dry leaves of the plant were purchased and authenticated. Oil extraction was done using Hydro-distillation method. Clinical isolates of Candida from denture wearers were speciated using CHROMagar. Well Diffusion test was used to confirm the antifungal activity. Hydro-distillation & Maceration methods of extraction were compared. MIC/MFC was determined using CSLI guidelines. Infra-Red Spectroscopy was used to identify the active functional group.
RESULTS: O.vulgare showed 30±3mm of zone of inhibition as against 19mm for fluconazole. The suitable extraction method was Hydro-distillation. MIC & MFC were found to be 0.024% and 0.097% respectively which was much lesser than for fluconazole (0.25%). The active functional group had chemically similar structure as Carvacrol, usually found in antifungal herbs.
CONCLUSION: Within the limitations of the study, it was concluded that (a)O.vulgare is anticandidal for clinical isolates of oral Candida, (b) Hydro-distillation is an effective method as compared to Maceration (c) MIC & MFC are much lower than that of fluconazole (d) the major functional group was structurally similar to Carvacrol.
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