Origanum Vulgare Essential Oil Affects Pathogens Causing Vaginal Infections.
Author Information
Karaman M1, Bogavac M2, Radovanović B2, Sudji J3, Tešanović K1, Janjušević L1.
1University of Novi Sad, Faculty of Sciences, Trg D. Obradovica 2, 21000, Novi Sad, Serbia.
2University of Novi Sad, Faculty of Medicine, Hajduk Veljkova 3, 21000, Novi Sad, Serbia.
3Institute of Occupational Health Novi Sad, Futoška 121, 21000, Novi Sad, Serbia.
Abstract
AIMS: This study aimed to investigate the application of Origanum vulgare L. essential oil (EO) as an alternative antimicrobial agent against vaginal infections.
METHODS AND RESULTS: Microdilution methods were applied for detection of minimal inhibitory (MIC) and minimal bactericidal/fungicidal MBC (MFC) concentration on 15 clinical strains originated from women with symptoms of vaginal infection. Optical density determination (OD) was used for detection of Candida growth rate. Staining with DAPI was used to detect the influence of EO on nuclear condensation and fragmentation, while brine shrimp bioassay was used to determine the toxicity of EO. Chemical composition analysis was done using GS-MS. According to MIC and MBC (MFC) values, the most susceptible strains to EO were: E. coli 1, E. coli 2, S. aureus 3 and C. albicans1-3. Inhibition of C. albicans filamentation was detected at 0.45 μl ml-1.
CONCLUSIONS: Obtained inhibition (%) from Candida growth curves point to a shorter period of time (24h) for determining IC50 as MIC and IC99 as MFC value. These values could be recommended as valid parameters for faster detection of EO effectiveness to Candida isolates.
SIGNIFICANCE AND IMPACT OF STUDY: Examination of O. vulgare EO potential as a main antimicrobial constituent within vaginaletes in gynecological practice.
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